Leopard x zebra (mother blue, father gold) yields ordinary zebra danios with stronger goldlines (resulting fish are fertile too)
RT-PCR was used to produce partial zebrafish matrilin-1 cDNA. Primers were designed according to genomic sequences deposited in the databases (). PCR were carried out according to the manufacturer's instructions using AmpliTaq polymerase (Applied Biosystems). In the case of nested and semi-nested PCRs, primer pairs designed against sequences inside PCR fragments were employed in a second PCR step. PCR fragments were cloned into pBluescript II using the Rapid Ligation Kit (Roche).
For many years scientists have been searching to identify the photoreceptor molecules that render zebrafish cells sensitive to light and to elucidate how they function to reset clock genes. There is supporting evidence for two possibilities, oxidative stress and opsins. Most data supporting oxidative stress suggest that flavin-containing oxidases are the light sensors responsible for circadian photoentrainment in zebrafish cells , ; however their functional role has yet to be demonstrated. Some studies have suggested that opsins such as Tmt may be the elusive photopigment , ; however, our results imply that Opn4 (or at least an opsin that shows some resemblance to the signaling pathway of melanopsin) may underpin photoentrainment in the zebrafish. Strengthening this possibility, light sensitivity of the cavefish Phreatichthys andruzzii clock is lost due to mutations in tmt and opn4m-2, and is rescued after transfection with zebrafish tmt and opn4m-2 . Since Tmt signaling has been proved to be via Gi/G0 , our data on blue light signaling through phosphoinositide cascade point to Opn4.
Cobalt Blue Zebra Cichlid - Aquarium Fish
Cobalt Blue Zebra Cichlid | Tim's Tropical Fish
The Cobalt Blue Zebra originated in deep rocky waters of Lake Malawi in Africa. It is also known as the Cobalt Blue Mbuna and the Cobalt Zebra. Thescientific name is Metriaclima callainos, but see the discussion below aboutMetriaclima estherae. The word “Mbuna” means “rock fish”, which refers to its habitat. The Cobalt Blue Zebra has a light blue color applied consistently over the entirebody with no faint zebra banding. The species is very similar and includes a blue version which can bedifferentiated by the occurrence of faint horizontal banding.In the basement at the Panum-building at the University of Copenhagen, Denmark, you will find a little piece of India. Everyday, the sun slowly rises around 8 AM and sets again around 10 PM. The humidity is high and the room temperature is a constant 26° C while the blue water is 28° C. The only ones taking a swim in the windowless room though, are the approx. 10,000 zebra fish that fill the approx. 1.000 aquariums lining the walls. Clean water and proper pH are important in maintaining an mbuna tank. Although some recommend using underground filters, external filters and bio-wheels simultaneously to maintain quality, my experience is that underground filtersare not suitable for mbunas. They build nests by picking up rocks in theirmouth and moving them, so underground filters are quickly exposed in an mbuna tank. Mbunas are very territorial, aggressive fish, even moreso than Haplochromis Cichlids like the , so they should only be kept with other mbunas, A grouping including more than 10mbunas is recommended as this will spread out aggressive behavior. The tank should be 75 – 100 gallons and should have rocks and hidingspaces to provide escape from aggression of other tankmates. Thereshould be smooth surfaced gravel for them to pick up in their mouths and move. Mbunas enjoy plants, but willuproot them because they constantly rearrange the substrate. Cobalt Blue Zebras will eat flake foods, spirulina flakes and cichlid pellets. They should not be fed live worms or live brine shrimp, as these can bloat and kill the fish.Love the 'ocelot' pattern mixed with long fin golden zebras. They go beautifully and easily with baby clown loaches, horse faced loaches, dojo loaches, yo yo loaches- you get my point, in a black water heavily planted SE Asian biotope or mixed with a pair of electric blue ram, school of diamond tetra or similar build characin and a dwarf bnp- I'm currently in love with my newly acquired Panama red bristle nose pair. I stumbled across this mixed black water biotope on accident while using a 40b to acclimate my small amazon inspired and southeast Asian inspired from one connected aquaponic system to their upgraded large tanks (smallest is 125g largest is 350g= 8 more planters for my garden). In my acclimation 40b I keep water sprite, ludwiga palustrus, ludwiga repens, Java moss and patches of crypt w. 'tropica' with the rams in a secure spot behind a water lily bulb and driftwood, diamond tetra schooling in the middle, these guys darting out and about in the floating water sprite, it was quite eye catching. I've noticed that my gold zebra have turned from yellow and white to an almost salmon and metallic gold and my diamond tetra are prettier than I ever pictured them when I first put them in their new set up. These little bitty surface show pieces are incredibly over looked because a lot of people don't realize how absolutely stunning they look when their natural habitat is replicated and their natural diet simulated. I would definitely suggest these guys to anyone starting out on their first nature scape inspired 30+ gallon tank as they are very adaptable and peaceful providing just enough surface motion and sparkle when paired with floating plants to bring out shy centerpiece fish out of hiding with out taking over the show! Perfect for beginners and experts and everyone in between. From: Ony